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中西α-半乳糖苷酶(黑曲霉) 2000U 爱尔兰 型号:JKY/E-AGLAN库号:M315599

北京中西远大科技有限公司
所在地:北京 
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外观 白色液体悬浮
比重〜1.5
溶解度 易溶于水
pH值 6.0
气味 无
悬浮状液体
稳定性 稳定 十年或以上(冰箱)


成分
名称 比例
α-半乳糖苷酶(黑曲霉)0.4%
硫酸铵45%
叠氮钠0.02%
水54.5%


性能 
1。电泳纯度 
- 单波段的SDS -凝胶电泳(兆瓦= 97,000) 
- 单主要波段电聚焦(π= 4.2)。 
2。比活度和水平的其他活动 
所有活动都是在pH 4.5和40℃糖苷酶测定的活动使用适当的 
硝基苯甙(10毫米)。内切聚糖酶的活动和活动的低聚糖 
在确定适当的基片(10毫克/毫升),并使用尼尔森/索姆吉 
减少糖的程序。活动单位之一,是需要的酶量释放 
1 micromole产品(如对硝基苯酚每分钟)在pH 4.5和40℃ 
基板酶实测比活度 
(U / mg蛋白) 
p-NP-α-半乳糖1 -半乳糖苷酶880 
p-NP-β-半乳糖ß -半乳糖苷酶0.1 
p-NP-α-葡萄糖苷1 -葡萄糖苷酶0.001 
p-NP-β-葡萄糖苷ß -葡萄糖苷酶5.0 
p-NP-β-木糖苷ß -木糖苷酶0.1 
p-NP-β-Mannoside ß -甘露0.01 
p-NP铝,阿糖胞苷基地阿拉伯糖苷酶0.001 
角豆半乳甘露聚糖内切- 1 ,4 -β-甘露聚糖酶“0.001 
蔗糖转化酶0.08 
1,蔗果三糖外切Inulinanase 0.05 
1,1 - Kestotetraose外切Inulinanase 0.05 
果聚糖(聚合物)外切Inulinanase 0.01 

3。理化性质 
***适pH:4.5-5.0。 pH值稳定性:4.0-8.0。 
温度远舰:60℃(在pH 5.0)。温度稳定性:60℃以上不稳定 
4。储存条件 
这种酶是作为一个提供0.02%叠氮化钠硫酸铵中止和 
应贮存在4℃在缓冲区解散后,酶应存放在 
冻结状态的聚丙烯容器之间使用。



α-Galactosidase (A. niger)
EC 3.2.1.22 
CAZY Family: GH36 
From A. niger. Highly purified. In 3.2 M ammonium sulphate.
Specific activity: >500 U/mg (40°C, pH 4.5, p-nitrophenyl α-D-galactopyranoside. 
Contaminants: β-mannanase and β-mannosidase <0.001%. This enzyme is very active on raffinose, stachyose and verbascose. 
Stable at 4°C for > 4 years.


Content: 2,000 Units

EC Number: 3.2.1.22


Appearance White liquid suspension
Specific Gravity ~ 1.5
Solubility in Water Readily soluble
pH Value 6.0
Odour None
Form Liquid suspension.
Stability Stable in a refrigerator for ten or more years
Ingredients Name CAS Proportion
a-Galactosidase (Aspergillus niger) 0.4%
Ammonium Sulphate 45%
Sodium Azide 0.02%
Water 54.5%

PROPERTIES
1. ELECTROPHORETIC PURITY
- Single band on SDS-gel electrophoresis (MW=97,000)
- Single major band on Isoelectric focusing (pI=4.2).
2. SPECIFIC ACTIVITY AND LEVEL OF OTHER ACTIVITIES
All activities are at pH 4.5 and 40°C. Glycosidase activities were measured using the appropriate
p-nitrophenyl glycoside (at 10 mM). endo-Glycanases activity and activity on oligosaccharides
were determined with the appropiate substrate (at 10 mg/ml) and using the Nelson/Somogyi
reducing-sugar procedure. One Unit of activity is the amount of enzyme required to release
one micromole of product (e.g. p-nitrophenyl) per min at pH 4.5 and 40°C.
SUBSTRATE ENZYME MEASURED SPECIFIC ACTIVITY
(U/mg protein)
p-NP-a-Galactoside a-Galactosidase 880
p-NP-ß-Galactoside ß-Galactosidase 0.1
p-NP-a-Glucoside a-Glucosidase 0.001
p-NP-ß-Glucoside ß-Glucosidase 5.0
p-NP-ß-Xyloside ß-Xylosidase 0.1
p-NP-ß-Mannoside ß-Mannosidase 0.01
p-NP-a-L-arabinoside a-L-arabinofuranosidase 0.001
Carob Galactomannan endo-1,4-ß-Mannanase <0.001
Sucrose Invertase 0.08
1-Kestose exo-Inulinanase 0.05
1,1-Kestotetraose exo-Inulinanase 0.05
Fructan (polymer) exo-Inulinanase 0.01
3. PHYSICOCHEMICAL PROPERTIES
pH Optima: 4.5-5.0. pH Stability: 4.0-8.0.
Temp Optima: 60°C (at pH 5.0). Temp Stability: Unstable above 60°C.
4. STORAGE CONDITIONS
The enzyme is supplied as an ammonium sulphate suspension in 0.02% sodium azide and
should be stored at 4°C. On dissolution in buffer, the enzyme should be stored in the
frozen state in a polypropylene container between use

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